Primary Hematopoietic and Lymphoid Tissue ›› Bone Marrow ›› Normal

Mature B-cells


Click Here for Full Size

---

› Microscopic Features:

• Slightly (1.5x) larger than a mature RBC
• High nuclear to cytoplasmic ratio but with
• Round mature Nucleus (clumped chromatin)
• Nucleoli are absent
• Cytoplasm is scant, light blue and lacks granules

› Normal % blood-PB, marrow-BM, lymphoid tissue-LN:

• PB: **** (2nd most common lymphocyte in blood after T-cells)
• BM: Rare scattered
• LN: Mainly distriibuted in Follicular areas (Germinal Center and Mantle zone)

› May Resemble:

• Mature T-cells (can't distinguish T-cell and B-cell based on morphology)
• Reactive lymphocyte [Compare]
• Monocyte [Compare]

› Differential Diagnoses:

If increased in blood, Lymph node or bone marrow: Infection Neoplastic (mature B-cell lymphoma or leukemia such as CLL)

› Classic Immunophenotype:

• CD19+
• CD20+
• CD22+
• TdT-
• CD34-

› Cartoon Image:


Click and drag

for direct comparison
› Misc:

• Morphologically you cannot distinguish mature T-cells from mature B-cells