Blood/Blood Cells and Cellular Components ›› Neutrophils ›› Normal

Segmented Neutrophil*


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Additional Image 1 Additional Image 2

› Microscopic Features:

• 2-3x larger than a mature RBC
• Low nuclear to cytoplasmic ratio (more cytoplasm than nucleus)
• Nucleus is mature and divided into 3 to 5 lobes connected by thin chromatin filaments
• Nucleoli are absent
• More cytoplasm with only secondary granules
• Secondary (specific) granules are neutrophilic (lilac)

› Normal % blood-PB, marrow-BM, lymphoid tissue-LN:

• PB: Many (most common WBC in blood)
• BM: Scattered
• LN: Rare to None

› May Resemble:

• HyperSegmented Neutrophil [Compare]
• Pseudo-Pelger Huet Neutrophil [Compare]
• Band Neutrophil [Compare]
• Neutrophil with toxic granules [Compare]
• Metamyelocyte [Compare]
• Myelocyte [Compare]
• Promyelocyte [Compare]
• Monocyte [Compare]

› Differential Diagnoses:

Increased in: Infections (especially bacterial infections) Myeloproliferative disorders (e.g. CML and CNL) Leukemoid reaction Drug-induced

› Classic Immunophenotype:

• CD34-
• CD117-
• HLA-DR-
• CD10+
• CD13+
• CD33+
• CD15+
• CD11b+
• CD16+
• MPO+

› Cartoon Image:


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› Misc:

• Normal segmentation is 3-5 lobes while Hypersegmentation is >5 lobes and hypo segmentation is <3 lobes