Primary Hematopoietic and Lymphoid Tissue ›› Bone Marrow ›› Normal

Segmented Neutrophil*

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Additional Image 1 Additional Image 2

Microscopic Features:
  • 2-3x larger than a mature RBC
  • Low nuclear to cytoplasmic ratio (more cytoplasm than nucleus)
  • Nucleus is mature and divided into 3 to 5 lobes connected by thin chromatin filaments
  • Nucleoli are absent
  • More cytoplasm with only secondary granules
  • Secondary (specific) granules are neutrophilic (lilac)
Normal % blood-PB, marrow-BM, lymphoid tissue-LN:
  • PB: Most common WBC in blood (~55-70%)
  • BM: Scattered (~5-10 %)
  • LN: Rare to None
May Resemble: Differential Diagnoses:

Increased in:
Infections (especially bacterial infections)
Myeloproliferative disorders (e.g. CML and CNL)
Leukemoid reaction due to other causes (e.g. stress)
Post-GCSF therapy

Classic Immunophenotype:
  • CD34-
  • CD117-
  • HLA-DR-
  • CD13+
  • CD33+
  • CD15+
  • CD11b+
  • CD16+
Cartoon Image:

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for direct comparison

  • Normal segmentation is 3-5 lobes while Hypersegmentation is >5 lobes

Content Editors/Website Administrators:
Hooman H. Rashidi, MD; John C. Nguyen, MD