Primary Hematopoietic and Lymphoid Tissue ›› Bone Marrow ›› Lymphoid ›› B-cells ›› Normal
Mature B-cells
›Microscopic Features:
- Slightly (1.5x) larger than a mature RBC
- High nuclear to cytoplasmic ratio but with
- Round mature Nucleus (clumped chromatin)
- Nucleoli are absent
- Cytoplasm is scant, light blue and lacks granules
›Normal % blood-PB, marrow-BM, lymphoid tissue-LN:
- PB: **** (2nd most common lymphocyte in blood after T-cells)
- BM: Rare scattered
- LN: Mainly distriibuted in Follicular areas (Germinal Center and Mantle zone)
›May Resemble:
- Mature T-cells (can't distinguish T-cell and B-cell based on morphology)
- Reactive lymphocyte
- Monocyte
›Differential Diagnoses:
If increased in blood, Lymph node or bone marrow:
Infection
Neoplastic (mature B-cell lymphoma or leukemia such as CLL)
Infection
Neoplastic (mature B-cell lymphoma or leukemia such as CLL)
›Classic Immunophenotype:
- CD19+
- CD20+
- CD22+
- TdT-
- CD34-
›Cartoon Image:
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for direct comparison
for direct comparison
›Misc:
Morphologically you cannot distinguish mature T-cells from mature B-cells